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dc.contributor.author Reid, William D. K.
dc.contributor.author Close, Andrew J.
dc.contributor.author Humphrey, Suzanne
dc.contributor.author Chaloner, Gemma
dc.contributor.author Lacharme-Lora, Lizeth
dc.contributor.author Rothwell, Lisa
dc.contributor.author Kaiser, Pete
dc.contributor.author Williams, Nicola J.
dc.contributor.author Humprey, Thomas J.
dc.contributor.author Wigley, Paul
dc.contributor.author Rushton, Stephen P.
dc.date.accessioned 2016-02-16T16:05:02Z
dc.date.available 2016-02-16T16:05:02Z
dc.date.issued 2016-03-16
dc.identifier doi:10.5061/dryad.7h3v1
dc.identifier.citation Reid WDK, Close AJ, Humphrey S, Chaloner G, Lacharme-Lora L, Rothwell L, Kaiser P, Williams NJ, Humprey TJ, Wigley P, Rushton SP (2016) Cytokine responses in birds challenged with the human food-borne pathogen Campylobacter jejuni implies a Th17 response. Royal Society Open Science 3: 150541.
dc.identifier.uri http://hdl.handle.net/10255/dryad.108720
dc.description Development of process orientated understanding of cytokine interactions within the gastrointestinal tract during an immune response to pathogens requires experimentation and statistical modelling. The immune response against pathogen challenge depends on the specific threat to the host. Here, we show that broiler chickens mount a breed-dependent immune response to Campylobacter jejuni infection in the caeca by analysing experimental data using frequentist and Bayesian structural equation models (SEM). SEM provides a framework by which cytokine interdependencies, based on prior knowledge, can be tested. In both breeds important cytokines including pro-inflammatory interleukin (IL)-1β, , IL-4, IL-17A, interferon (IFN)-γ and anti-inflammatory IL-10 and transforming growth factor (TGF)-β4 were expressed post-challenge. The SEM revealed a putative regulatory pathway illustrating a T helper (Th)17 response and regulation of IL-10, which is breed-dependent. The prominence of the Th17 pathway indicates the cytokine response aims to limit the invasion or colonization of an extracellular bacterial pathogen but the time-dependent nature of the response differs between breeds.
dc.relation.haspart doi:10.5061/dryad.7h3v1/1
dc.relation.isreferencedby doi:10.1098/rsos.150541
dc.relation.isreferencedby PMID:27069644
dc.subject gastrointestinal tract
dc.subject Th1 and Th17 response
dc.subject structural equation model
dc.subject bacterial pathogen
dc.subject gamma-delta T lymphocytes
dc.subject inflammation
dc.title Data from: Cytokine responses in birds challenged with the human food-borne pathogen Campylobacter jejuni implies a Th17 response
dc.type Article
dwc.ScientificName Campylobacter jejuni
dc.contributor.correspondingAuthor William Reid
prism.publicationName Royal Society Open Science

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Description These cytokine data are based on total RNA samples taken from caecal tissue of commercial broilers, which were held under laboratory conditions. The birds were challenged with the pathogen Campylobacter jejuni. The results are expressed as fold changes in corrected target gene expression in infected birds relative to controls.
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