Show simple item record Plough, Louis V.
dc.coverage.spatial United States 2018-02-10T19:47:13Z 2018-02-10T19:47:13Z 2018-02-08
dc.identifier doi:10.5061/dryad.6fm12
dc.identifier.citation Plough LV (2018) Fine-scale temporal analysis of genotype-dependent mortality at settlement in the Pacific oyster Crassostrea gigas. Journal of Experimental Marine Biology and Ecology 501: 90-98.
dc.description Settlement and metamorphosis mark a critical transition in the life cycle of marine invertebrates, during which substantial mortality occurs in both field and laboratory settings. Previous pair-crossing experiments with the Pacific oyster Crassostrea gigas have revealed significant selective or genotype-dependent mortality around the metamorphic transition, but the fine-scale nature and timing of this mortality is not known, particularly whether it occurs before, during or after metamorphosis. In this laboratory study, microsatellite marker segregation ratios were followed daily throughout the settlement and metamorphosis of an F2 cross of the Pacific oyster to examine the fine-scale patterns of genotype dependent mortality at this transition and whether settlement timing (early vs. late) might be under genetic control and affect inference of genotype dependent mortality. Settlement occurred over nine days (day 18 to day 27 post-fertilization) with 68% of individuals settling either early (day 19) or late (day 24). Tracking the survival of spat for 40 days after initial settlement revealed almost no mortality and thus no appreciable genetic mortality. Temporal genetic analysis revealed that 3/11 loci exhibited genotype dependent mortality around the metamorphic transition, one of which (Cg205) was followed throughout settlement and metamorphosis. Alternative temporal patterns of strong selection against each homozygous genotype at Cg205 revealed possible defects in both the competency pathway (inability to initiate metamorphosis) and the morphogenesis pathway (mortality during the metamorphic transition). Quantitative trait locus (QTL) mapping of settlement timing identified three individual and one epistatic QTL with significant genetic effects on this trait (29% of the variance explained in total); however, two of these loci were linked to markers exhibiting selective mortality at metamorphosis, potentially confounding their apparent association with settlement timing. Overall, the results of this study highlight the complex nature of mortality and behavior during settlement and metamorphosis in oysters and suggest that endogenous sources of mortality at settlement may play an important role in the recruitment dynamics of oysters and possibly other broadcast spawning marine invertebrates.
dc.relation.haspart doi:10.5061/dryad.6fm12/1
dc.relation.isreferencedby doi:10.1016/j.jembe.2018.01.006
dc.subject recruitment
dc.subject larval
dc.subject metamorphosis
dc.subject life history
dc.subject genetic load
dc.title Data from: Fine-scale temporal analysis of genotype-dependent mortality at settlement in the Pacific oyster Crassostrea gigas
dc.type Article
dwc.ScientificName Crassostrea gigas
dc.contributor.correspondingAuthor Plough, Louis V.
prism.publicationName Journal of Experimental Marine Biology and Ecology

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Title Genotype Data for oyster spat (C. gigas) from early and late settlement
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Description This excel sheet contains microsatellite marker genotypes for oyster spat settling on day 19 or day 24. Genotypes were called by eye (and compared to parent genotypes) on a ABI 377 prism instrument using the Genescan software. These data form the basis of the QTL mapping analyses and chi-square comparisons of early vs. late settlement in Plough 2018. Column A contains the individual Spat ID, and columns C through BK contain the genotype data. Microsatellite marker names are prefixed by "Cg" and are presented in pairs of columns for each marker: one for spat settling on day 19, the other for day 24 (Row 1). Row 2 contains the cross-type of the parents (e.g. abxab) and Row 3 contains the C. gigas linkage group (LG) information for a given marker (LGs 1-10).
Download Plough JEMBE Spat genotype data DRYAD.xlsx (24.87 Kb)
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